Description; Application; 5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.; 5-Bromo-2′-deoxyuridine has been used: • as a supplement to study the effect of electroconvulsive seizures on hippocampal neurogenesis[3] • as a supplement to study the loss-of-gene function in adult zebrafish heart[4] • in T lymphocyte proliferation assay{133]; Packaging; 1, 5, 100 g in glass bottle; 100, 250, 500 mg in glass bottle; Biochem/physiol Actions; 5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation,[2] because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells.[1] Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.; Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.; Features and Benefits; This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.; Legal Information; LOPAC is a registered trademark of Sigma-Aldrich Co. LLC