Description; Application; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt has been used: • as a component in β-glucuronidase (GUS) assay buffer for histochemical GUS assay[2] • to prepare X-Gluc or GUS staining solution[3][4] • to confirm GUS expression in samples by X-gluc staining[5]; Chromogenic substrate for β-glucuronidase (GUS) gene detection.; Packaging; 10, 25, 100 mg in poly bottle; Biochem/physiol Actions; 5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colorless substrate for β-glucuronidase enzyme, generates a blue color at the end of the reaction.[1]