Description_x000D_ General description_x000D_ 5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides._x000D_ Biochem/physiol Actions_x000D_ 5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis._x000D_ 5'-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5'−monophosphates to nucleosides with a substrate preference of AMP. Native 5'-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5'-nucleotidase has many functions in vivo including the generation of extracellular adenosine._x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C._x000D_ Physical form_x000D_ Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol.