Description General description A potent, ATP-competitive, and specific inhibitor of casein kinase II (IC50 = 6 µM). Reported to inhibit insulin-stimulated nuclear and cytosolic p70S6 kinase in CHO cells. Accentuates premature termination of transcription. Has been used to inhibit RNA polymerase II transcription which may be dependent on casein kinase II. Inhibits germinal vesicle breakdown in bovine oocytes. Induces G1/S cell cycle arrest. Accentuates premature termination of transcription close to the promoter. Useful for isolation of RNA sequences near promoter sites that are responsible for mRNA formation. Useful for reversible superinduction of human fibroblast cultures. Potent, ATP-competivie and specific inhibitor of casein kinase II (IC50 = 6 µM). Has been used to inhibit RNA polymerase II transcription, which may be dependent on casein kinase II. Inhibits germinal vesicle breakdown in bovine oocytes. Packaging 50 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: no Primary Target casein kinase II Product competes with ATP. Reversible: no Warning Toxicity: Standard Handling (A) Preparation Note May require warming (47°C) to achieve complete dissolution. Reconstitution Room temperature (+20°C). Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Ethanol stock solutions are stable for up to 6 weeks at 4°C or for up to 3 months at -20°C. Other Notes Kim., S.J., and Kahn, C.R. 1997. Biochem. Biophys. Res. Commun. 234, 681. Farin, C.E., and Yang, L. 1994. Mol. Reprod. Dev.37, 284. Giardina, C., and Lis, J.T. 1993. J. Biol. Chem. 268, 23806. Zandomeni, R., et al. 1986. J. Biol. Chem.261, 3414. Mittleman, B., et al. 1983. J. Mol. Biol. 165, 461. Zandomeni, R., et al. 1983. J. Mol. Biol. 167, 561. Zandomeni, R., et al. 1982. Proc. Natl. Acad. Sci. USA79, 3167. Stewart, M.W., et al. 1977. J. Gen. Virol.37, 221.; Description