Description Application 7-Amino-4-methylcoumarin has been used: • to determine cathepsin B like- activity • as a substrate for leucine aminopeptidase (LAP) • in chymotryptic assay • as a standard to detect the activation of caspase-3 during • sanguinarine-induced damage • protection by human DEAD-box DDX3 Packaging 1 g in poly bottle 250, 500 mg in poly bottle Biochem/physiol Actions 7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors. Preparation Note Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL). Other Notes Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.