Description_x000D_ General description_x000D_ Acetate, the ionized form of acetic acid, is a critical metabolite in fatty acid and carbohydrate metabolism. The majority of acetate is utilized as the conjugate to coenzyme A (CoA), acetyl-CoA. Acetyl-CoA is a precursor to fatty acid and cholesterol synthesis and an important starting compound for the citric acid cycle. Acetyl-CoA also serves as a precursor for the synthesis of acetylcholine and as the donor for acetyl groups for post-translational acetylation reactions of histone and non-histone proteins. Acetate is generated from the metabolism of carbohydrates, amino acids, fatty acids, and ethanol._x000D_ The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present._x000D_ Application_x000D_ Acetate colorimetric assay kit has been used to determine acetate concentrations calorimetrically._x000D_ Suitability_x000D_ Suitable for the detection of acetate in serum, plasma, animal tissues (liver, kidney, muscle, heart etc.), cell culture (adherent or suspension cells) and food._x000D_ Principle_x000D_ The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.