Description_x000D_ Application_x000D_ Acetyl coenzyme A is suitable in CAT reporter assay for the measurement of chloramphenicol acetyltransferase by incubating the cell extract with 0.1 μCi [14C] chloramphenicol and 0.5mM acetyl-CoA._x000D_ An essential cofactor in enzymatic acetyl transfer reactions._x000D_ Packaging_x000D_ 1, 5 mg in glass bottle_x000D_ 10, 25, 100 mg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Alterations in the gene encoding acetyl-CoA have been linked to Alzheimer′s disease and mild cognitive impairment. Defects in the gene cause myasthenic syndrome associated with episodic apnea._x000D_ Acetyl-CoA is an essential cofactor and carrier of acyl groups in enzymatic acetyl transfer reactions. It is formed either by the oxidative decarboxylation of pyruvate in mitochondria, by the oxidation of long-chain fatty acids, or by the oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound for the citric acid cycle (Kreb′s cycle). It is also a key precursor in lipid biosynthesis, and the source of all fatty acid carbons. Acetyl-CoA positively regulates the activity pyruvate carboxylase. It is a precursor of the neurotransmitter acetylcholine. Histone acetylases (HAT) use Acetyl-CoA as the donor for the acetyl group use in the post-translational acetylation reactions of histone and non-histone proteins._x000D_ Physical properties_x000D_ This moisture-sensitive powder should be stored desiccated at −20°C._x000D_ Preparation Note_x000D_ Prepared enzymatically_x000D_ Reconstitution_x000D_ Acetyl CoA is soluble in deionized water at 100 mg/mL. Acetyl CoA is stable in neutral and moderately acidic solutions but will hydrolyze in alkaline and strongly acidic solutions. Aqueous solutions stored in single-use aliquots are stable for up to two weeks at −20°C and up to 6 months at −80°C._x000D_ Other Notes_x000D_ For more more technical information and a complete list of Coenzyme A deriviatives visit the Acyl Transfer Reagents Resource.