Description_x000D_ General description_x000D_ Molecular Weight: 280 kDa_x000D_ Isoelectric Point: 5.5_x000D_ Extinction Coefficient: E1% = 18.0 (280 nm)_x000D_ Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines._x000D_ Application_x000D_ The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride._x000D_ Packaging_x000D_ 10000 units in poly bottle_x000D_ 500, 2000 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid._x000D_ Unit Definition_x000D_ One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C._x000D_ Physical form_x000D_ Lyophilized powder containing Tris buffer salts_x000D_ Preparation Note_x000D_ This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution._x000D_ Analysis Note_x000D_ The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.