Description_x000D_ Specificity_x000D_ Recognizes ASIC2. The epitope used for the immunogen is specific for ASIC2 and is not similar to any other known protein. The epitope is not specific for ASIC2b (MDEG2) splice variant._x000D_ SPECIES REACTIVITIES: It is expected that the antibody will also react with mouse due to immunogen sequence homology (100%). Reactivity with other species has not yet been tested._x000D_ Immunogen_x000D_ Epitope: neuronal_x000D_ Highly purified peptide corresponding to residues 2-18 of rat or human ASIC2 (Accession Q16515)._x000D_ Application_x000D_ Anti-Acid Sensitive Ion Channel 2 Antibody, neuronal is an antibody against Acid Sensitive Ion Channel 2 for use in WB._x000D_ Research Category_x000D_ Neuroscience_x000D_ Research Sub Category_x000D_ Ion Channels & Transporters_x000D_ Western blotting: 1:200 using ECL on rat brain membranes._x000D_ Dilutions should be made using a carrier protein such as BSA (1-3%)._x000D_ Optimal working dilutions must be determined by the end user._x000D_ Physical form_x000D_ Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min)._x000D_ Storage and Stability_x000D_ Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Analysis Note_x000D_ Control_x000D_ CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The antigen should be reconstituted with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user._x000D_ Other Notes_x000D_ Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.