Description_x000D_ General description_x000D_ Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel._x000D_ Application_x000D_ Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 5% stacking gel and 7% resolving gel for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)._x000D_ These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide._x000D_ Preparation Note_x000D_ Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.