Description_x000D_ General description_x000D_ Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel._x000D_ Application_x000D_ Acrylamide/Bis-acrylamide, 30% solution has been used:_x000D_ • in the preparation of polyacrylamide gel for photobleaching experiment_x000D_ • as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution_x000D_ • in the preparation of polyacrylamide gels during western blot analyses_x000D_ These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide._x000D_ Preparation Note_x000D_ Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.