Description_x000D_ General description_x000D_ Alcohol dehydrogenases (ADH) are a family of enzymes that catalyzes the conversion of alcohols to aldehydes, with the concomitant reduction of NAD+ to NADH. In humans, there are nine isozymes of ADH, with the majority of ADH activity occurring in the liver. ADH family members are the primary enzymes involved in alcohol detoxification. Genetic variations in ADH enzymes result in differences in ADH activity and tolerances for alcohol, and may regulate susceptibility to alcoholism._x000D_ Application_x000D_ Alcohol Dehydrogenase Activity Assay Kit has been used to determine the activity of alcohol dehydrogenase._x000D_ Suitability_x000D_ Suitable for the detection of alcohol dehydrogenase in a variety of samples including tissue, cells and serum._x000D_ Principle_x000D_ The Alcohol Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring ADH activity in a variety of samples. ADH activity is determined using ethanol as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of ADH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at 37 °C.