Description_x000D_ General description_x000D_ Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria._x000D_ Application_x000D_ Alginate Lyase has been used:_x000D_ • in the pre-processing of enzyme_x000D_ • to de-encapsulate cells_x000D_ • to digest Pseudomonas exopolysaccharide to facilitate electroporation_x000D_ Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture ._x000D_ Packaging_x000D_ 100 mg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol._x000D_ Breaks down alginate or alginic acid to smaller molecules and reduces viscosity._x000D_ Unit Definition_x000D_ One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C_x000D_ Preparation Note_x000D_ Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH._x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer