Description_x000D_ General description_x000D_ The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate._x000D_ Application_x000D_ Alkaline Phosphatase Diethanolamine Activity Kit has been used:_x000D_ • in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts_x000D_ • for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells_x000D_ • for determining ALP activity in the bone metastasis model of lung cancer_x000D_ • for measuring ALP levels in colon tissue homogenates_x000D_ Suitability_x000D_ Suitable for the measurement of alkaline phosphatase activity in a variety of samples_x000D_ Principle_x000D_ Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.