Description_x000D_ General description_x000D_ The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications._x000D_ Application_x000D_ Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications._x000D_ Caution_x000D_ Stable at least one year at 2-8 °C._x000D_ Physical form_x000D_ Ready to use_x000D_ Preparation Note_x000D_ Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.