Description_x000D_ General description_x000D_ Chemical structure: polyene_x000D_ Packaging_x000D_ 1, 5 g in glass bottle_x000D_ 50, 100, 500 mg in glass bottle_x000D_ Application_x000D_ Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium._x000D_ Biochem/physiol Actions_x000D_ Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast._x000D_ Packaging_x000D_ Solubilized Amphotericin B is packaged in amber glass bottle, with the contents inside an inserted fused cone._x000D_ Components_x000D_ This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride._x000D_ Caution_x000D_ Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years._x000D_ Preparation Note_x000D_ Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution._x000D_ Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize._x000D_ It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL)._x000D_ Other Notes_x000D_ Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.