Description General description Full length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening. Packaging 5 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Unit Definition Kinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP. Physical form In 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5. Reconstitution Following reconstitution, aliquot and freeze (-70°C). Other Notes Foretz, M., et al. 2005. Diabetes54, 1331. Viollet, B., et al. 2003. Biochem. Soc. Trans.31, 216.