Description Specificity Recognizes AT1 Receptor (Angiotensin II receptory type 1, AGTR1). The immunogen sequence is identical in canine. Immunogen Purified peptide from the N-terminus of human AT1 Receptor (Accession number P30556). Application Anti-AT1 Receptor Antibody detects level of AT1 Receptor & has been published & validated for use in FC, IC, IH & WB. Research Category Neuroscience Research Sub Category CNS Control of Metabolism Hormones & Receptors Western blot: 1:200-1:500 using ECL on rat liver, rat kidney, mouse heart and mouse kidney lysate. Immunohistochemistry on rat brain sections. Immunocytochemistry: 1:100 Flow cytometry, indirect: 5-10 µg of antibody /1 x 106 cells Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal working dilutions must be determined by the end user. Physical form Format: Purified ImmunoAffinity Purified Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). Storage and Stability Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Analysis Note Control Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.