Description_x000D_ General description_x000D_ Zyxin (UniProt: Q15942; also known as Zyxin-2) is encoded by the ZYX gene (Gene ID: 7791) in human. Zyxin is an evolutionarily conserved LIM domain protein that acts as an adhesion plaque protein concentrated at sites of cell adhesion and is widely expressed. It has been implicated in cell adhesion, cell motility, and signaling. Zyxin is reported to associate with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family of cytoskeletal regulators and plays a role in cytoskeletal dynamics and signaling. Zyxin contains three LIM zinc binding domains (aa 384-443; 444-503; and 504-570), which act as a negative regulator of Zyxin-VASP complexes. Also, it contains four proline rich ActA repeats (aa 64-77; 94-108; 115-121; and 127-137) that are known to interact directly with Ena/VASP proteins. Loss of Zyxin results in inability to thicken stress fibers in response to mechanical stimuli. (Ref.: Hoffman LM et al (2003). Mol. Cell Biol. 23(1); 70-79; Moody JD et al. (2009). Biochem. Biophys. Res. Commun. 378(3), 625-628; Yoshigi et al (2005). J. Cell Biol. 171(2), 209-215.)._x000D_ Specificity_x000D_ This rabbit polyclonal antibody detects Zyxin in human, mouse, and chicken cells. It targets an epitope within 20 amino acids from the C-terminal half._x000D_ Immunogen_x000D_ Epitope: unknown_x000D_ KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Zyxin._x000D_ Application_x000D_ Research Category_x000D_ Cell Structure_x000D_ Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Zyxin in A431 cell lysate._x000D_ Immunofluorescence Analysis: A representative lot detected Zyxin in newborn mouse skin. Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2012). Mol Biol Cell. 23(10):1846-59)._x000D_ Western Blotting Analysis): A representative lot detected Zyxin in mouse lung, mouse embryo fibroblasts and in human Ewing sarcoma cells.(Chaturvedi, A., et. al. (2014). Mol Biol Cell. 25(18):2695-709; Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2012). Mol Biol Cell. 23(10):1846-59; Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82)._x000D_ Immunocytochemistry Analysis: A representative lot detected Zyxin at focal adhesions in mouse fibroblasts and in 4T1 cells with ATG7 shRNA or ATG5 shRNA (Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82; Sharifi, M.N., et. al. (2016). Cell Rep. 15(8):1660-72)._x000D_ Quality_x000D_ Evaluated by Western Blotting in NIH3T3 cell lysate._x000D_ Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Zyxin in NIH3T3 cell lysate._x000D_ Target description_x000D_ ~75 kDa observed; 61.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s)._x000D_ Physical form_x000D_ Rabbit polyclonal antiserum with 0.05% sodium azide._x000D_ Unpurified_x000D_ Storage and Stability_x000D_ Stable for 1 year at -20°C from date of receipt._x000D_ Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance._x000D_ Other Notes_x000D_ Concentration: Please refer to lot specific datasheet._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.