Description General description Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells. Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial). Antibody Target Gene Symbol: ACTA2 • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta • Hu Entrez ID: 59 (Related Antibodies: 113200) • Rat Entrez ID: 81633 This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin. Immunogen Human Smooth Muscle a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin. Application Frozen Sections (1:50-1:75) Immunoblotting (see comments) Paraffin Sections (1:50-1:75) Physical form In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5. Packaging 500 μL in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Negative Control Fibroblasts, striated muscle, or myocardium Positive Control Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary Other Notes Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution. Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532. Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315. Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.