Description_x000D_ General description_x000D_ Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein ._x000D_ Specificity_x000D_ Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human._x000D_ Immunogen_x000D_ Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH._x000D_ Application_x000D_ Anti-l-Afadin antibody produced in rabbit has been used in:_x000D_ • western blotting_x000D_ • immunofluorescence experiments_x000D_ • immunocytochemistry_x000D_ • immunohistochemistry_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide_x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.