Description_x000D_ General description_x000D_ Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome._x000D_ Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations._x000D_ This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits._x000D_ Immunogen_x000D_ Epitope: within the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)_x000D_ dinitrophenylated proteasomes_x000D_ Application_x000D_ _x000D_ Immunoblotting (1:1000, chemiluminescence)_x000D_ Immunoprecipitation (not recommended)_x000D_ ELISA (see comments)_x000D_ Immunofluorescence (see application references)_x000D_ Immunocytochemistry (see application references)_x000D_ Immunohistochemistry (see application references)_x000D_ Frozen Sections (see application references)_x000D_ Packaging_x000D_ 100 μL in Plastic ampoule_x000D_ Warning_x000D_ Toxicity: Highly Toxic (H)_x000D_ Analysis Note_x000D_ Positive Control_x000D_ Human placental tissue_x000D_ Other Notes_x000D_ Immunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems._x000D_ Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA94, 2939.