Description General description Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine. Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells. Specific • Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications • Flow Cytometry • Frozen Sections • Immunoblotting • Immunofluorescence • Paraffin Sections Reliable • Generates reproducible results. • Clone 162 33 D3 has been used in over 50 publications. This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine. Immunogen 5-methylcytosine conjugated to ovalbumin Application ELISA (0.1 µg/ml) Flow Cytometry (1 µg/ml) Frozen Sections (5-10 µg/ml) Immunoblotting (5 µg/ml) Immunofluorescence (1 µg/ml) Paraffin Sections (5-10 µg/ml) Radioimmunoassay (0.1 µg/ml) Southwestern Blot (5 µg/ml) Physical form In PBS, pH 7.4. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control NIH3T3 cells Other Notes Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems. Ehrlich, M. 2002. Oncogene21, 5400. (Review) Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review) Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983. Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856. Taddei, A., et al. 2001. Nat. Cell Biol.3, 114. Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251. De Capoa, A., et al. 1999. FASEB J.13, 89.