Description General description Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry. Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein. Recognizes the ~70-75 kDa AFP protein in HepG2 cells. Immunogen A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein Application ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Physical form In PBS, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 100 μg in Glass bottle Warning Toxicity: Regulatory Review (Z) Analysis Note Negative Control 293T cells Positive Control HepG2 cells Other Notes For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.