Description General description Anti-Angiopoietin-2, rabbit polyclonal, recognizes the ~55 kDa non-glycosylated and the ~70 kDa glycosylated, secreted Ang-2 protein. It is validated for ELISA, WB, IF, and IHC. Immunoaffinity purified rabbit polyclonal antibody. Recognizes both the ~55 kDa nonglycosylated and the ~70 kDa glycosylated forms of angiopoietin-2. Recognizes both the ~55 kDa non-glycosylated and the ~70 kDa glycosylated, secreted Ang-2 protein in cell culture medium. Does not cross-react with Ang-1. Antibody Target Gene Symbol: ANGPT2 • Target Synonym: AGPT2, ANG II, ANG-2, ANGIOPOIETIN, ANGIOPOIETIN 2, Angiotensin 2, Angrp • Entrez Gene Name: angiopoietin 2 • Hu Entrez ID: 285 • Mu Entrez ID: 11601 • Rat Entrez ID: 89805 Immunogen a synthetic peptide corresponding to a 21 amino acid cytoplasmic sequence at the N-terminus of mouse angiopoietin-2 Application ELISA (0.1-1 µg/ml) Immunoblotting (1-10 µg/ml) Immunofluorescence (2-20 µg/ml) Immunohistochemistry (2-20 µg/ml) Packaging 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Other Notes Maisonpierre, P.C., et al. 1997. Science 277, 55. Davis, S., et al. 1996. Cell 87, 1161. Suri, S. et al. 1996. Cell 87, 1171. The immunogenic peptide sequence is 71% (15/21 amino acids) conserved in the human angiopoietin-2 protein. Does not cross-react with angiopoietin-1. Variables associated with assay conditions will dictate the optimal working dilution.