Description_x000D_ General description_x000D_ ARP2 is part of a complex with 7 subunits called ARP2/3. ARP2 is an actin-related protein that function to regulate the actin cytoskeleton._x000D_ Anti-ARP2 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FMS96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant human ARP2._x000D_ Mouse Monoclonal Anti-ARP2 antibody binds to human, canine, rat, and mouse ARP2, 40-45kDa._x000D_ Immunogen_x000D_ recombinant human ARP2_x000D_ Application_x000D_ Anti-ARP2 antibody, Mouse monoclonal has been used in:_x000D_ • Immunoblotting_x000D_ • Immunocytochemistry_x000D_ • Enzyme linked immuosorbent assay_x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Actin-related protein 2 (ARP2) is a part of ARP2/3 complex and play a key role in actin filament formation. It mediates changes in the polymerization state of actin filaments. The Wiskott-Aldrich Syndrome protein (WASP) regulates ARP2/3 complex of proteins. The ARP2/3 complex and its activator spinocerebellar ataxia, autosomal recessive 2 (Scar2) are involved in Golgi polarization in NIH 3T3 cells. However in primary astrocytes, Golgi polarization involves neither actin cytoskeleton or Arp2/3 complex nor any WASP-family of proteins. In a different model, it was found that the ARP2/3 complex was necessary for neutrophil chemotaxis and phagocytosis. By genetic and loss of function studies, it was demonstrated that Scar is the major regulator of the ARP2/3 complex rather than the WASP family of proteins, the latter having more restricted roles in specific cellular events.