Description_x000D_ General description_x000D_ Monoclonal Anti-c-erbB-4 (erb-b2 receptor tyrosine kinase 4) (HER-4) (mouse IgG1 isotype) is derived from the HER4-36 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-4 from transfected cells. The human ErbB-4 oncogene (also known as HER4) is a 180–185 kDa receptor that binds to a family of isoforms, collectively known as neuregulins. This gene belongs to epidermal growth factor receptor family and is expressed mainly in several breast carcinoma cell lines, heart, brain, cerebellum, pituitary and in normal skeletal muscle._x000D_ Immunogen_x000D_ extracellular domain of human erbB-4 from transfected cells._x000D_ Application_x000D_ Anti-c-erbB-4 antibody, Mouse monoclonal has been used in immunoprecipitation. It has also been used in studies on receptor turnover, phosphorylation, ligand binding and induction of differentiation of cultured breast cancer cells._x000D_ Monoclonal anti-c-erbB-4 antibody can be used in immunoprecipitation (4-8 μg/test) using RIPA lysate of cultured CB4 cells (CHO cell line transfected with erbB-4). It can also be used in microarray._x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ The epidermal growth factor receptor (ErbB/HER) family of transmembrane receptor tyrosine kinases significantly participates in defining cell lineage during mesenchyme-epithelial inductive processes and in the neuro-muscular and glia-Schwann cells interactions. It plays a crucial role as a cell surface receptor for neuregulins. It can also bind with erbB -2 to forms heterodimers and can modulate ligand affinity. The catalytic domain is important for the intrinsic tyrosine kinase activity and shows greater degree of homology. Certain ligands and receptors of the family contribute to a relatively virulent phenotype of some human tumors; most notable are carcinomas of secretory epithelia. ErbB-4 also binds to related group of molecules, termed neuregulin 2 (NRG2)(which also binds to erbB-3) and NRG3 (which binds exclusively to erbB-4). Two other ligands, betacellulin and the heparin-binding EGF-like growth factor, bind to both erbB-1 and erbB-4. ErbB-4 forms heterodimers with erbB-2, resulting in the modulation of its ligand affinity.