Description_x000D_ General description_x000D_ Anti-c-ErbB2/c-Neu (Ab-4), mouse monoclonal, clone 7.16.4, recognizes ~185 kDa c-ErbB2 in overexpressing B104-1-1 cells. It is validated for WB, IP, ICC, frozen sections, & growth inhibition assay._x000D_ Purified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-neu protein._x000D_ Recognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells._x000D_ Immunogen_x000D_ B104-1-1 cells overexpressing rat c-ErbB2/c-Neu_x000D_ Epitope: Within the extracellular domain_x000D_ Human_x000D_ Application_x000D_ _x000D_ Frozen Sections (5 µg/ml, see application references)_x000D_ Immunoblotting (see application references)_x000D_ Immunocytochemistry (5 µg/ml)_x000D_ Immunoprecipitation (1 µg/ml, see application references)_x000D_ Neutralization Studies (not recommended, see application references)_x000D_ Physical form_x000D_ In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5._x000D_ Packaging_x000D_ 100 μg in Plastic ampoule_x000D_ Please refer to vial label for lot-specific concentration._x000D_ Warning_x000D_ Toxicity: Standard Handling (A)_x000D_ Analysis Note_x000D_ Negative Control_x000D_ Fisher rat embryo cells_x000D_ Positive Control_x000D_ B104-1-1 cells_x000D_ Other Notes_x000D_ Stains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems._x000D_ Zhang, H., et al. 1999. Exp. Mol. Pathol.67, 15._x000D_ Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol.17, 1031._x000D_ Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA85, 5389._x000D_ Van De Vijver, M.J., et al. 1988. New Engl. J. Med.319, 1239._x000D_ DiFiore, P.P., et al. 1987. Science237, 178._x000D_ Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA84, 8498._x000D_ Slamon, D.J., et al. Science235, 177._x000D_ Varley, J.M., et al. 1987. Oncogene1, 423._x000D_ Bargmann, C.I., et al. 1986. Nature319, 226._x000D_ Yamamoto, T., et al. 1986. Nature319, 230._x000D_ Blick, M., et al. 1984. Blood64, 1234._x000D_ Schwab, M., et al. 1984. Cold Spring Harbor Laboratory2, 215.