Description General description Anti-Calreticulin (405-417), rabbit polyclonal, recognizes the ~63 kDa calreticulin in heat-shocked HeLa cells. It is validated for WB, ICC, IHC, and IP. Protein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein. Recognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells. Immunogen Human a synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH Application Immunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent) Immunocytochemistry (see comments) Immunoprecipitation (see comments) Immunohistochemistry (see comments) Physical form In PBS, 50% glycerol, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 50 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Other Notes Does not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution. Janicki, S., et al. 1997. J. Cell Biol. 139, 485. Wada, I., et al. 1997. EMBO J. 16, 5420. Nauseef, W.F., et al. 1995. J. Biol. Chem.270, 4741. Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603. Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.