Description General description Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay. Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein. Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells. Immunogen Human a recombinant protein consisting of human calreticulin fused to maltose binding protein Application Enzyme Immunoassay (0.1 µg/ml) Immunoblotting (0.5 µg/ml, colorimetric; see application references) Immunocytochemistry (see application references) Immunohistochemistry (see application references) Flow Cytometry (see application references) Physical form In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells Other Notes Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution. Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741. Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603. Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.