Description General description Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies. Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein. Recognizes the ~72 kDa CD64 protein (FcR1 receptor). Immunogen Human human rheumatoid synovial fluid cells/monocytes Application Flow Cytometry (10 µg/ml) Frozen Sections (see comments) Immunoblotting (see comments) Immunoprecipitation (see comments) Neutralization Studies (see comments) Physical form In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5. Reconstitution Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles. Packaging 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Negative Control HPB-MLT Positive Control 4937 cells Other Notes Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453. The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.