Description General description Cell division control protein 42 (UniProt: P19073; also known as Cdc42, Suppressor of RHO3 protein 2) is encoded by the CDC42 (also known as SRO2, TLR229C, L8083.13) gene (Gene ID: 850930) in yeast (Saccharomyces cerevisiae). Cdc42 is a member of the Rho GTPase family that regulates multiple signaling pathways involved in actomyosin organization, cell proliferation, and maintenance of cell polarity in most eukaryotic cell types. Cdc42 signaling process involves guanine nucleotide exchange factors (GEFs), guanine nucleotide dissociation inhibitors (GDIs), and GTPase-activating proteins (GAPs). In yeast, generation and maintenance of robust Cdc42 polarity promotes membrane expansion during bud formation and it has been shown that Cdc42 is dynamically maintained at the polarity cap through its continuous cycling between the polarity cap and internal pools. Cdc42 is highly polarized during bud emergence and is specifically required for exocytosis at this stage. Cdc42 is synthesized with a propeptide region (aa 189-191), which is cleaved off in the mature form. The mature form contains three nucleotide (GTP)-binding sites. (Ref.: Wu, H., and Brennwald, P. (2010) Mol. Cell Biol. 30(21); 5207-5217). Specificity Clone 28-10 specifically detects Cell division control protein 42 (Cdc42). Immunogen GST-tagged full-length recombinant yeast Cdc42. Application Anti-Cdc42, clone 28-10, Cat. No. MABN2485, is a highly specific mouse monoclonal antibody that targets Cell division control protein 42 (Cdc42) and has been tested for use in Immunocytochemistry and Western Blotting. Research Category Neuroscience Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17). Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17). Target description ~21 kDa observed; 21.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Physical form Format: Purified Protein G purified Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cdc42 in HeLa cell lysate. Other Notes Concentration: Please refer to lot specific datasheet.