Description General description CUGBP Elav-like family member 4 (UniProt: Q9BZC1; also known as CELF-4, Bruno-like protein 4, CUG-BP- and ETR-3-like factor 4, RNA-binding protein BRUNOL-4) is encoded by the CELF4 (also known as BRUNOL4) gene (Gene ID: 56853) in human. CELF-4 is a RNA-binding protein that is implicated in the regulation of pre-mRNA alternative splicing. It is ubiquitous in its distribution, but is strongly expressed in the cerebellum, hippocampus, amygdala, temporal and frontal cortex, and frontal lobes. It mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. It is shown to specifically activate exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. CELF-4 is reported to promote exclusion of both the smooth muscle and non-muscle exons in actinin pre-mRNAs. It binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. The N-terminal region (aa 1-298) is considered to be sufficient for its RNA-binding and MSE-dependent splicing activity. CELF-4 contains contain two N-terminal RNA recognition motif (RRM) domains (aa 54-135 and 152-232) and one C-terminal RRM domain (aa 404-479). Five isoforms of CELF-4 have been described that are produced by alternative splicing. Specificity Clone N446/80 specifically detects CUGBP Elav-like family member 4 (CELF4). It targets an epitope with in 86 amino acids from the N-terminal region. It does not react with CELF6. Immunogen A recombinant fragment corresponding to 86 amino acids from the N-terminal region of human CUGBP Elav-like family member 4 (CELF-4). Epitope: N-terminus Application Anti-CELF4, clone N446/80, Cat. No. MABN2418, is a highly specific mouse monoclonal antibody that targets CUGBP Elav-like family member 4 and has been tested for use in Western Blotting. Research Category Neuroscience Target description ~46 kDa observed; 51.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Physical form Format: Purified Protein G purified Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Western Blotting in human hippocampus tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CELF4 in human hippocampus tissue lysate. Other Notes Concentration: Please refer to lot specific datasheet.