Description General description Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein. Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP. Immunogen a recombinant protein consisting of full-length human CHIP fused to a His•Tag sequence Application Immunoblotting (1:200-1:1000) Immunofluorescence (1:250) Immunoprecipitation (1:20) Physical form Undiluted serum. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 100 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Analysis Note Positive Control COS-7 cells Other Notes May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems. Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847. Demand, J., et al. 2001. Curr. Biol.11, 1569. Connell, P., et al. 2001. Nat. Cell Biol.3, 93. Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938. Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100. Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.