Description General description Claudin-5, a transmembrane protein deleted in velo-cardio-facial syndrome (TMVCF), seems to be involved in endothelial tight junctions. Claudin-5 has been exclusively found in the cell-cell borders of endothelial cells. Lower claudin-5 levels in sinusoidal endothelial cells could lead to their dysfunction during liver injuries. Levels of claudin-5 in tumor vessels could be a potential marker for hepatocellular carcinoma prognosis. The loss of claudin-5 function has been linked to carcinogenesis. Specificity This antibody recognizes Claudin-5 at the cytoplasmic domain. Immunogen Epitope: Cytoplasmic Domain KLH-conjugated linear peptide corresponding to human Claudin-5 at the cytoplasmic domain. Application Anti-Claudin-5 Antibody detects level of Claudin-5 & has been published & validated for use in WB, IH(P). Immunohistochemistry Analysis: A 1:800 dilution from a representative lot detected Clausin-5 in prostate tissue. Research Category Cell Structure Research Sub Category Adhesion (CAMs) Target description ~17 kDa observed. Molecular weights at ~17 kDa and 62 kDa may be observed depending on phosphorylation and glycosylation effects (Ishizaki, T., et al. (2003). Experimental Cell Research. 290:275–288). Physical form Affinity Purfied Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage and Stability Stable for 1 year at 2-8°C from date of receipt. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Quality Evaluated by Western Blot in human lung tissue lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected Claudin-5 in 10 µg of human lung tissue lysate. Analysis Note Control Human lung tissue lysate Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.