Description_x000D_ General description_x000D_ Immunoprecipitation is a method by which a protein can be specifically purified from a complex mixture of proteins using a specific antibody and a matrix that binds the antibody. The matrix bound protein can then be separated from the mixture by centrifugation. IP followed by SDS-PAGE and immunoblotting are routinely used in a variety of applications to measure the molecular masses of protein antigens, study protein interactions, determine specific enzymatic activity, monitor protein post-translation modifications, and determine the presence and quantity of proteins. The IP technique also enables the detection of rare proteins, which otherwise would be difficult to detect, since immunoprecipitation can concentrate them to as much as 10,000-fold._x000D_ Application_x000D_ Specially designed to allow maximal recovery of c-Myc-tagged proteins in the immunoprecipitates. The whole process is performed in mini-spin columns, instead of in microcentrifuge tubes, thus combining convenient washing of the antigen-antibody-bound beads with minimal variability between experiments._x000D_ Anti-c-Myc immunoprecipitation kit was used in the identification of epithelial stromal interaction 1 as a novel effector downstream of Krüppel-like factor 8 in breast cancer invasion and metastasis. It was also used to study the CD81 interaction with the T cell receptor to suppress signaling._x000D_ Legal Information_x000D_ CelLytic is a trademark of Sigma-Aldrich Co. LLC_x000D_ Features and Benefits_x000D_ • Spin columns ensures more consistent results_x000D_ • Anti-c-Myc Affinity Gel ensures high specific binding to c-Myc-tagged fusion protein_x000D_ • CelLytic™ M Cell Lysis Reagent for rapid and efficient mammalian cell lysis