Description Application Anti-Cytokeratin 14 Antibody, clone LL002 is an antibody against Cytokeratin 14 for use in WB, IH(P). Suitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formalin. Western blotting Optimal working dilutions must be determined by the end user. Physical form Format: Purified The monoclonal is presented at a concentration of 100 μg/mL in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. Quality Tested Other Notes feature_concentration_valuePlease refer to lot specific datasheet.