Description General description Purified mouse monoclonal antibody. Recognizes the DEFB4 protein. Recognizes the DEFB4 protein. This Anti-DEFB4 Mouse mAb (4C4) is validated for use in ELISA, Immunoblotting for the detection of DEFB4. Immunogen A recombinant polypeptide corresponding to amino acids 24-65 of human DEFB4, expressed as a GST fusion protein Application ELISA (see comments) Immunoblotting (1-5 µg/ml) Physical form In PBS, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Warning Toxicity: Regulatory Review (Z) Other Notes For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.