Description_x000D_ General description_x000D_ Purified mouse monoclonal antibody. Recognizes the ~20-22 kDa DHFR protein._x000D_ Recognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells._x000D_ This Anti-DFHR Mouse mAb (2B10) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of DFHR._x000D_ Immunogen_x000D_ A recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein_x000D_ Application_x000D_ _x000D_ _x000D_ _x000D_ ELISA (see comments)_x000D_ Immunoblotting (1-5 µg/ml)_x000D_ Immunocytochemistry (10 µg/ml)_x000D_ Physical form_x000D_ In PBS, pH 7.2._x000D_ Reconstitution_x000D_ Following initial thaw, aliquot and freeze (-20°C)._x000D_ Warning_x000D_ Toxicity: Regulatory Review (Z)_x000D_ Analysis Note_x000D_ Negative Control_x000D_ 293T cells_x000D_ Positive Control_x000D_ HeLa cells, PC-12 Cells_x000D_ Other Notes_x000D_ For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution._x000D_ Vodenicharov, M.D., et al. 2010. The EMBO Journal29, 3007._x000D_ Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol.295 H2178.