Description General description Anti-Dopamine D5 Receptor, rabbit polyclonal, recognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC. Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~49 kDa D5 dopamine receptor protein. Recognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors. Immunogen Human a synthetic peptide (LPPGSNGTAYC) corresponding to amino acids 2-10 of human dopamine D₅ receptor, conjugated to carrier protein Application ELISA (see comments) Flow Cytometry (1:100, see application references) Immunoblotting (1:200) Immunohistochemistry (1:2500) Physical form Undiluted serum. Reconstitution Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Packaging 100 μL in Plastic ampoule Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Rat brain Other Notes For immunohistochemistry, a 1:2500 dilution of the reconstituted antiserum demonstrated staining of specific cells in various regions of rat brain sections, including Cortex layer 5 and parafascicular nucleus. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution. McKenna, F., et al. 2002. J. Neuroimmunol.132, 34.; Description