Description General description Anti-EDN3, mouse monoclonal, clone 2A6-2A4, recognizes the ~26 kDa EDN3 protein in LNCaP cells. it is validated for use in ELISA and Western blotting. Purified mouse monoclonal antibody. Recognizes the ~26 kDa EDN3 protein. Recognizes the ~26 kDa EDN3 protein in LNCaP cells. Immunogen Full-length, recombinant human EDN3 (aa 1-239), expressed as a GST fusion protein Application ELISA (see comments) Immunoblotting (1-5 µg/ml) Physical form In PBS, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Warning Toxicity: Regulatory Review (Z) Analysis Note Negative Control 293T cells Positive Control LNCaP cells Other Notes For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.