Description_x000D_ General description_x000D_ Eph family of tyrosine kinase receptors mediates cell-cell attachment and aggregation by binding to specific ligands, ephrins. The Eph family consists of 14 structurally related receptors that are either membrane linked or are transmembrane with overlapping affinity for ligands. The transmembrane receptor Ephrin-B2 signals through its cognate receptor EphB4. The signalling via EphB4 receptor ensures is crucial in the initial stages of vasculogenesis in marking specific arterial and venous domains. Ephrin-B2 is also essential in the development stages for neural crest formation, angiogenesis, vascular cell assembly, migration and synapse development in central nervous system_x000D_ Anti-Ephrin-B2 recognizes human Ephrin-B2 protein (~56 kDa)._x000D_ Ephrin-B2 is a member of the ephrin-B (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases. Ephrins are divided into ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-B2 binds the tyrosine kinase receptors EphB4 and EphA3 and is expressed by arteries whereas its receptor tyrosine kinase EphB4 is expressed by veins._x000D_ Immunogen_x000D_ human Ephrin-B2 recombinant protein. The human protein shares 97% homology with the mouse protein._x000D_ Application_x000D_ Anti-Ephrin-B2 antibody may be used for immunoblotting at a working concentration of 0.5-1.0 μg/ml using whole cell lysates of HEK-293T cells over-expressing human Ephrin-B2. It is also suitable for immunohistochemistry of formalin-fixed, paraffin-embedded human skin sections._x000D_ Physical form_x000D_ Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Biochem/physiol Actions_x000D_ Ephrin-B2 and EphB4 are essential for proper development of the cardiovascular system. It is implicated in regulating erythropoiesis. Mutations in these genes are linked to embryonic lethality, defects in the angiogenic remodeling of the peripheral vasculature and defective myocardial trabeculation in the heart.