Description General description Anti-FASN, mouse monoclonal, clone 3F2-1F3, recognizes FASN protein in 293 and HeLa cells and in human breast carcinoma tissue. It is validated for ELISA, WB, ICC, and IHC (paraffin sections). Purified mouse monoclonal antibody. Recognizes the FASN protein. Recognizes the FASN protein in 293 and HeLa cells and human breast carcinoma tissue. Immunogen Full-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein Application ELISA (see coments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (10 µg/ml) Physical form In PBS, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Warning Toxicity: Regulatory Review (Z) Analysis Note Positive Control 293 cells, HeLa cells, Human breast carcinoma tissue Other Notes For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution. Kristiansen, G., et al. 2010. Br. J. Cancer102, 1736.