Description General description Anti-FGF8, mouse monoclonal, clone 2A10, recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells. It is validated for use in ELISA and Western blotting. Purified mouse monoclonal antibody. Recognizes the ~30-35 kDa FGF8 protein. Recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells. Immunogen Full-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein Application ELISA (see comments) Immunoblotting (1-5 µg/ml) Physical form In PBS, pH 7.2. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Warning Toxicity: Regulatory Review (Z) Analysis Note Positive Control PC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells Other Notes For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.