Description_x000D_ General description_x000D_ Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. Fibronectin (FN), also known as Cold-insoluble globulin (CIG), is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN1 gene 6. In vertebrates, two types of Fibronectin are present: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue._x000D_ Specificity_x000D_ Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human and mouse origin._x000D_ Immunogen_x000D_ Fibronectin isolated from human plasma._x000D_ Application_x000D_ The antibody may be used in various immunochemical techniques including Immunoblotting (∼220-260kDa)1, ELISA, Immunofluorescence, Immunohistochemistry and Immunoprecipitation._x000D_ Physical form_x000D_ Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative._x000D_ Biochem/physiol Actions_x000D_ The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue._x000D_ Other Notes_x000D_ In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.