Description_x000D_ General description_x000D_ Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent._x000D_ Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide_x000D_ Immunogen_x000D_ DYKDDDDK_x000D_ Application_x000D_ Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins._x000D_ Browse additional application references in our FLAG® Literature portal._x000D_ Physical form_x000D_ Suspension in buffered saline containing azide as preservative and 50% glycerol_x000D_ Legal Information_x000D_ ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ FLAG is a registered trademark of Sigma-Aldrich Co. LLC