Description; General description; Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support. form: solution pH 7.4, containing 15 mM sodium azide concentration: 3.0-5.0 mg/mL; Application; IB, IF, IP, FACS, ELISA Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.; Packaging; polypropylene screw cap vial; Preparation Note; Dilute the antibody solution from 0.5-10 ug/mL in specified buffer; Legal Information; ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC; FLAG is a registered trademark of Sigma-Aldrich Co. LLC