Description_x000D_ General description_x000D_ FN3K catalyzes phosphorylation of fructosamines formed by glycation, the nonenzymatic reaction of glucose with primary amines followed by Amadori rearrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and result in deglycation of glycated proteins. Human and mouse FN3K share 86% amino acid identity. GENE Purified FN3K catalyzes ATP-dependent phosphorylation of a synthetic fructosamine, 1-deoxy-1-morpholinofructose (DMF). Recombinant mouse and human FN3K, expressed in E. coli, catalyze phosphorylation of DMF, fructoselysine, fructoseglycine, and fructose in order of decreasing affinity. They also phosphorylate glycated lysozyme, but not unmodified lysozyme. In addition to ATP, FN3K can utilize GTP, CTP, and UTP as phosphate donors._x000D_ Immunogen_x000D_ FN3K (NP_071441, 284-319)_x000D_ This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human FN3K._x000D_ Physical form_x000D_ Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.