Description_x000D_ General description_x000D_ _x000D_ ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies._x000D_ Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them._x000D_ Learn more about ZooMAb here._x000D_ Specificity_x000D_ Clone 4B20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects GRB2-associated-binding protein 1 (Gab1). It targets an epitope within 30 amino acids from the C-terminal region._x000D_ Immunogen_x000D_ KLH-conjugated Linear Peptide corresponding to 30 amino acids from the C-terminal region of Human Gab1._x000D_ Application_x000D_ Anti-Gab1, clone 4B20 ZooMAb, Cat. No. ZRB1348, is a recombinant Rabbit monoclonal antibody that specifically targets GRB2-associated-binding protein 1 (Gab1) and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting._x000D_ Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Gab1 in fetal bovine heart endothelial (FBHE) cell lysate._x000D_ Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Gab1 in human small intestine tissue sections._x000D_ Affinity Binding Assay: A representative lot of this antibody bound Gab1 peptide with a KD of 4.0 x 10-6 in affinity binding assay._x000D_ Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user._x000D_ Target description_x000D_ GRB2-associated-binding protein 1 (UniProt: Q13480; also known as GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1; Gab1) is encoded by the GAB1 gene (Gene ID: 2549) in human. Gab1 is a scaffolding protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. It recruits PI-3 kinase and other effector proteins in response to the activation of many receptor tyrosine kinases (RTKs). Gab1 can be phosphorylated on at least 16 sites by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta, hepatocyte growth factor/scatter factor receptor, and epidermal growth factor receptor. Phosphorylation of Gab1 on tyrosine 627 and tyrosine 659 is shown to be critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. Tyrosine phosphorylation of Gab1 mediates interaction with several proteins that contain SH2 domains. Gab1 plays important roles in embryonic development and cell migration and is shown to be a critical component in the interaction between focal adhesion kinase and Met in cancer cell invasion. Gab1 is also reported to be a major target in linoleic acid (LA)-induced enhancement of tumorigenesis. Mutations in GAB1 gene are known to cause autosomal recessive deafness that is characterized by severe to profound hearing loss resulting from damage to the neural receptors of the inner ear or the nerve pathways to the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mattoon, DR., et al. (2004). BMC Biol. 2; 24; Mouradian, M., et al. (2014). Prostaglandins Leukot. Essent. Fatty Acids. 90(4); 105-115)._x000D_ Physical form_x000D_ Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL._x000D_ Reconstitution_x000D_ 300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type._x000D_ Storage and Stability_x000D_ Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws._x000D_ Disclaimer_x000D_ Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals._x000D_ Legal Information_x000D_ ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany