Description General description Purified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP). Recognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues. This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein. Immunogen Bovine Brain bovine GFAP Application ELISA (0.1-0.5 µg/ml) Immunoblotting (0.1-0.5 µg/ml) Frozen Sections (10-50 µg/ml) Immunoprecipitation (2-5 µg/ml) Paraffin Sections (10-50 µg/ml, see comments) Physical form In PBS. Reconstitution Following initial thaw, aliquot and freeze (-20°C). Packaging 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. Warning Toxicity: Standard Handling (A) Analysis Note Positive Control Human or rat brain tissues Other Notes Product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin′s-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution. Tohyama, T., et al. 1993. Am. J. Pathol.142, 871. Lee, V.M., et al. 1984. J. Neurochem. 42, 25.; Description